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random 9 mer primers  (New England Biolabs)


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    New England Biolabs random 9 mer primers
    Random 9 Mer Primers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/9+mer+primers/Random+Primer+9/pmc12421886-300-15-27
    Average 94 stars, based on 171 article reviews
    random 9 mer primers - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Modification:

    Article Title: The Evaluation of SHAPE-MaP RNA Structure Probing Protocols Reveals a Novel Role of Mn 2+ in the Detection of 2'-OH Adducts.
    Article Snippet: The concentration, integrity, and purity of modified mRNA samples were assessed using an Agilent Bioanalyzer 2100 with an RNA Nano 6000 kit (mRNA assay, Agilent Technologies, Inc., Santa Clara, CA, USA). .. A total of 200 ng of the modified RNA was subjected to reverse transcription with random 9-mer primers (New England Biolabs, Ipswich, MA, USA). .. Primers were annealed by incubation at 65 ◦C for 5 min. We have used the following protocols: SuperScript II Reverse Transcriptase (SSII, Thermo Fisher Scientific) with Mn2+ buffer (SSII-Mn2+), SuperScript IV Reverse Transcriptase (SSIV, Thermo Fisher Scientific Inc., Waltham, MA, USA) with Mn2+ buffer (SSIV-Mn2+), and a standard SSIV buffer (SSIV) and TGIRT-III Enzyme (InGex, St Louis, MO, USA) with a standard TGIRT buffer (TGIRT, InGex, St Louis, MO, USA)).

    Reverse Transcription:

    Article Title: The Evaluation of SHAPE-MaP RNA Structure Probing Protocols Reveals a Novel Role of Mn 2+ in the Detection of 2'-OH Adducts.
    Article Snippet: The concentration, integrity, and purity of modified mRNA samples were assessed using an Agilent Bioanalyzer 2100 with an RNA Nano 6000 kit (mRNA assay, Agilent Technologies, Inc., Santa Clara, CA, USA). .. A total of 200 ng of the modified RNA was subjected to reverse transcription with random 9-mer primers (New England Biolabs, Ipswich, MA, USA). .. Primers were annealed by incubation at 65 ◦C for 5 min. We have used the following protocols: SuperScript II Reverse Transcriptase (SSII, Thermo Fisher Scientific) with Mn2+ buffer (SSII-Mn2+), SuperScript IV Reverse Transcriptase (SSIV, Thermo Fisher Scientific Inc., Waltham, MA, USA) with Mn2+ buffer (SSIV-Mn2+), and a standard SSIV buffer (SSIV) and TGIRT-III Enzyme (InGex, St Louis, MO, USA) with a standard TGIRT buffer (TGIRT, InGex, St Louis, MO, USA)).



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